ANSR® for Salmonella

1. 12 strips of 8 cluster tubes in a rack, 1.2 mL
2. 12 strips of 8 reaction tubes, 200 µL, containing lyophilized ANSR for Salmonella reagents in 2 sealed foil pouches with desiccant pack
3. 12 strips of 8 permanent caps for reaction tubes
4. 1 bottle of lysis reagent suspension buffer, 60 mL
5. 3 vials containing lyophilized lysis reagents
6. 1 kit insert

Equipment Required

1. ANSR reader (Neogen item 9828)
2. Computer and software for connection to ANSR reader (Neogen item 9832)
3. 1 dual heater block with lysis block inserts for 1.2 mL cluster tubes, 80 ± 2°C and 37 ± 2°C (Neogen items 9386-DUAL48, 9829-48)
4. Pipettor, 100 – 1000 µL (Neogen item 9463)
5. Pipette tip rack, 100 – 1000 µL, sterile, 96 tips (Neogen item 9487)
6. Pipettor, 10 – 100 µL, 8-channel (Neogen item 9388)
7. Pipette tips, 100 µL, sterile, filtered, 96 tips (Neogen item 9389)
8. Vortex mixer, adjustable speed (Neogen item 9494)
9. Stomacher (optional)
10. 3 thermometers, traceable (Neogen item 9518)
11. Timer, 3-channel (Neogen item 9426)
12. Optional heater block with block insert for 0.2 mL reaction tubes, 56 ± 1°C (Neogen items 9386-48D, 9829-64)
13. Webcam (Neogen item WEBCAM)
14. ANSR ethernet cable (Neogen item 9835)
15. Pipette pump, 10 mL (Neogen item 9277)
16. Pippettes, sterile serological, 10 mL (Neogen item 8686)
17. 40-slot, 20 mm test tube rack, autoclavable (Neogen item 9553)
18. Pipettor, 20-200 µL (Neogen item 9276)

Other Materials Required

1. Stomacher-type bags for sample enrichment. Filtered bags are recommended (Neogen item 6827 or equivalent)
2. Graduated cylinder, 250 mL (Neogen item 9368 or equivalent)

Media Enrichment Broth Required

1. Novobiocin, sodium salt (Neogen item 7985 or equivalent)
2. Rappaport-Vassiliadis Broth (Neogen item 7512 or equivalent). For raw meat sample confirmation only.
3. Phosphate Buffered Saline (PBS) (Neogen item 8425 or equivalent)
4. Potassium iodide (Sigma no. 221945 or equivalent)
5. Iodine (Sigma no. 207772 or equivalent)

Enrichment Broth Required (Dependent on sample type)

1. ANSR for Salmonella Enrichment Broth 1 (Neogen item 9811) - use with processed meats and other processed foods with a moderate to high microbial loads such as hot dogs, raw almonds, peanut butter, black pepper and cocoa powder.
2. ANSR for Salmonella Enrichment Broth 2 (Neogen item 9812) - use with raw meat, poultry carcass rinses, raw frozen shrimp, raw leafy greens and flours including soy.
3. ANSR for Salmonella Enrichment Broth 3 (Neogen item 9813) - use with environmental (except poultry house samples) and low microbial loads such as oat cereal and ice cream.
4. ANSR for Salmonella Enrichment Broth 3B (Neogen item 9817) - use with dry pet food and pasteurized egg products.
5. Tetrathionate Broth Base, Hajna (TT Hajna) (Neogen item 7740A) - use with poultry house environmental samples.