Veratox® VIP for Walnut
SKU No.  700002601  | Catalog No.  8550
Top

Veratox® VIP for Walnut

SKU No.  700002601  |  Catalog No.  8550

  • Features an optimized antibody combination and extraction procedure for high specificity to all types of walnut protein, particularly further-processed walnut.
  • Highly sensitive, quantifying walnut protein down to 0.15 ppm, aligned with most up-to-date industry guidelines.
  • Rapid and easy to use, with a 30-minute time to result; a simple testing procedure consistent across sample types; and ready-to-use, non-hazardous reagents.
Learn more
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact us.
To order or for more information contact us.

Veratox® VIP (Veratox Improvement Platform) for Walnut Allergen offers enhanced quantitative analysis of very low levels of raw and further-processed walnut residues in a wide variety of food products, ingredients, and clean-in-place rinses. This product can also be used to screen environmental swabs.

Specifications
BrandVeratox® VIP
AnalyteWalnut
PlatformELISA
Result TypeQuantitative
Limit of Detection

.03 ppm walnut protein

Range of Quantitation0.15 ppm walnut - 3.75 ppm walnut
Testing Time30.00 Minutes
Storage Conditions

Store refrigerated

Quantity per Package48 tests
Package Weight1.00 lb
  1. 48 antibody-coated microwells
  2. 48 red-marked transfer wells
  3. 6 yellow-labeled bottles of 0, 0.15, 0.375, 0.75, 1.5, and 3.75 ppm walnut protein controls
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop Solution
  7. One bottle of 20x allergen extraction buffer (AEB). Each bottle is enough to prepare 5 L of extraction solution in distilled or deionized water (pH 7.4)
  8. 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  9. 200 g of extraction additive in a specimen cup
  1. Allergen Extraction Kit (Neogen item 8429)
    1. 20 disposable plastic extraction bottles
    2. 20 sample collection tubes (12 x 75 mm) with caps
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile swabs
    2. 100 dropper tips
  3. Extraction additive for chocolate samples only (Neogen item 8551)
  4. Shaker water bath capable of maintaining 60 ± 1°C with clamps to hold 250 mL disposable plastic bottles (Neogen items 9298, 9299)
  5. Whatman #4 filters or equivalent (Neogen item 9429)
  6. Centrifuge
  7. Pipettor, adjustable 50–200 μL (Neogen item 9276)
  8. Pipettor, 12-channel (Neogen item 9273)
  9. Pipette tips (Neogen item 9410, 9407, 9417)
  10. Timer (Neogen item 9426)
  11. Microwell reader with a 650 nm filter (Neogen item 9303)
  12. 1 L bottle to prepare washing solution (Neogen item 9472)
  13. 1 L heat safe bottle to prepare extract solution (Neogen item 9472)
  14. Paper towels or equivalent absorbent material
  15. Microwell holder (Neogen item 9402)
  16. Waterproof marker
  17. Wash bottle (Neogen item 9400)
  18. Distilled or deionized water
  19. 3 reagent boats for 12-channel pipettor (Neogen item 9435)
  20. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  21. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)

Additional Information

A Guide to Risk Assessment Using Food Allergen Reference Doses

Technology

The Veratox VIP for Walnut test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). This assay is validated to detect three primary allergenic proteins in walnuts. Walnut protein is extracted from samples with a buffer solution by shaking in a heated water bath, followed by centrifugation or filtration. Extracted walnut protein is sampled and added to antibody-coated wells (capture antibody), where it binds to the antibody during an incubation. Any unbound walnut protein is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound walnut protein. After a second wash, substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop Solution reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities (OD). The OD of the controls form a standard curve, and the sample OD are plotted against the curve to calculate the concentration of walnut protein.