Gabapentin Forensic ELISA Kit
SKU 番号 182419 | カタログ番号
- Highly sensitive
- Multiple matrices
- Large menu of tests available
| 仕様 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ブランド | Neogen® | ||||||||||||
| 分析物 | Gabapentin | ||||||||||||
| アプリケーション | 尿, 血, 口腔液, 法医学マトリックス | ||||||||||||
| 使用目的 | For the determination of trace quantities of Gabapentin and/or other metabolites in urine, blood, serum and oral fluid. Contact a Neogen representative for information on other matrices. | ||||||||||||
| 種 | ヒト, 研究動物 | ||||||||||||
| Assay Sample Size | Oral Fluid: 50 µL Blood, Serum, Urine: 10 µL | ||||||||||||
| プラットフォーム | ELISA | ||||||||||||
| 結果の種類 | 定性 | ||||||||||||
| マイクロプレート サイズ | 96 well microplate | ||||||||||||
| 感度 |
*Oral Fluid Buffer is sold separately. The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50. | ||||||||||||
| 合計アッセイ培養時間 | 75 minutes | ||||||||||||
| 薬剤パネル | 処方薬, 作業環境検査 | ||||||||||||
| 保管時条件 | Test kit 2-8°C; store controls -20°C | ||||||||||||
| 波長 | 450 nm with acid stop | ||||||||||||
| 1 パッケージ当たりの数量 | 96 wells | ||||||||||||
技術
ELISA Assay Theory
Neogen’s direct competitive ELISAs operate on the basis of competition between the horseradish peroxidase (HRP) enzyme conjugate and the analyte in the sample for a limited number of specific binding sites on the precoated microplate.
ELISA Assay Procedure
Samples, standards or calibrators are first added to the precoated antibody microplate. Next, the enzyme conjugate is added and the mixture is incubated at room temperature. During incubation, competition for binding sites on the microplate is taking place. The plate is then washed, removing all unbound material. The bound enzyme conjugate is detected by the addition of a TMB-based substrate.
ELISA test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650 nm or 450 nm if acid stop is used. The extent of color development is inversely proportional to the amount of analyte in the sample or standard.
ELISA Assay Test Principle Procedure
- Plates are precoated with the antibody. The plate is ready for use. DO NOT WASH.
- A sample or control is added to each well. Next the drug-enzyme conjugate is added and the mixture is incubated at room temperature.
- Wash the plate to remove all unbound materials.
- The bound materials now remain in the microplate.
- Add TMB substrate to each well and allow the color to develop.
- Qualitative results are obtained by measuring the absorbance reading at 650 nm or 450 nm if acid stop is used.
トレーニング
Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training.